Signalling

Part:BBa_K212004

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)


secretion signal peptide + GFPmut3b

This is a composite signalling part. The secretion signal peptide for beta-lactamase in S. epidermidis is ligated N-terminally to registry part BBa_E0040 (GFPmut3b). The signal peptide motif is used by the bacteria to target the protein for secretion through the Sec pathway. Any part may be put in place of GFP, but in its current state, the amount of secretion of any protein may be studied using confocal microscopy.

NOTE: This part only works for S. epidermidis ATCC 12228

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 760


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Categories
Parameters
None